Journal: STAR Protocols

Article Title: Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR

doi: 10.1016/j.xpro.2023.102445

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Article Snippet: Myc-Tag (9B11) Mouse mAb (PE Conjugate), use 1:300 dilution , Cell Signaling Technology , Cat# 3739S; RRID:AB_10889248.

Techniques: Purification, Virus, Recombinant, Software

Journal: eLife

Article Title: Registered report: Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF

doi: 10.7554/eLife.11999

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Article Snippet: Mouse α myc (9B11) (HRP conjugate) , Antibody , , , Cell Signaling Technology , 2040 , .

Techniques: Transfection, Plasmid Preparation, Western Blot, Membrane, Staining

Journal: eLife

Article Title: Registered report: Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF

doi: 10.7554/eLife.11999

Figure Lengend Snippet:

Article Snippet: Mouse α myc (9B11) (HRP conjugate) , Antibody , , , Cell Signaling Technology , 2040 , .

Techniques: Transfection, Plasmid Preparation, Transduction, Western Blot, Membrane, Staining

Journal: eLife

Article Title: Registered report: Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF

doi: 10.7554/eLife.11999

Figure Lengend Snippet:

Article Snippet: Mouse α myc (9B11) (HRP conjugate) , Antibody , , , Cell Signaling Technology , 2040 , .

Techniques: Transfection, Plasmid Preparation, Western Blot, Transduction, Membrane, Staining

Journal: Cell Death and Differentiation

Article Title: SIRT3 promotes lipophagy and chaperon-mediated autophagy to protect hepatocytes against lipotoxicity

doi: 10.1038/s41418-019-0356-z

Figure Lengend Snippet: SIRT3 suppresses lipogenesis in lipotoxic hepatocytes. a SCD1 protein level in vector and SIRT3OE hepatocytes treated with or without P/O mixture for 24 h. b SIRT3 and SCD1 protein levels in SIRT3 and SCD1 double overexpressed hepatocytes. The lipid content c and the cellular TG content d in SIRT3 and SCD1 double overexpressed hepatocytes. White: blank, vector; gray: blank, SIRT3OE; light gray: P/O, vector; black: P/O, SIRT3OE. Data are shown as mean ± S.D., n = 6, * p < 0.05, and *** p < 0.001, vector vs. SIRT3OE, ### p < 0.001 blank vs. P/O treatment. && p < 0.01, &&& p < 0.001, SCD1OE vs. vector. One-way ANOVA was used to calculate the p -values

Article Snippet: The plasmid of pCMV3-SCD1-N-Myc was purchased from Sino Biological (MG51311-NM, Wayne, PA, USA).

Techniques: Plasmid Preparation

Journal: Cell Death and Differentiation

Article Title: SIRT3 promotes lipophagy and chaperon-mediated autophagy to protect hepatocytes against lipotoxicity

doi: 10.1038/s41418-019-0356-z

Figure Lengend Snippet: Schematic of the role of SIRT3 in protecting hepatocytes against lipotoxicity. SIRT3 activates AMPK to enhance macroautophagy and CMA on LDs and promote LDs dispersion on detyrosinated MTs, inhibits SCD1 expression to suppress lipogenesis, and deacetylates LCAD to increase fatty acid oxidation in mitochondria, resulting in attenuation of lipotoxicity in hepatocytes

Article Snippet: The plasmid of pCMV3-SCD1-N-Myc was purchased from Sino Biological (MG51311-NM, Wayne, PA, USA).

Techniques: Expressing